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pinducer 20 egfp teadi  (Addgene inc)


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    Addgene inc pinducer 20 egfp teadi
    Pinducer 20 Egfp Teadi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pinducer+20+egfp+teadi/pInducer20+EGFP-TEADi+(Plasmid+%23140145)/bio_rxiv__64898__2025__12__11__693520-235-22-24
    Average 93 stars, based on 9 article reviews
    pinducer 20 egfp teadi - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Selection:

    Article Title: p53 and YAP/TAZ-TEAD activities determine metaplastic heterogeneity in pancreatic cancer
    Article Snippet: .. LentiCas9-blast selection and pUSEPB selection was performed with 10 μg/mL blasticidin for 7 days and 10 μg/mL puromycin for 7 days respectively. pInducer-20 EGFP-TEADi (Addgene, #140145) was transduced into PDEC and HPAC cells followed by five days of selection using 1 mg/mL geneticin/G418. cDNAs for wildtype and phospho-null YAP and TAZ and EGFP-TEADi were cloned out of pInducer-20 and into the retroviral backbone pBABE-puro for transduction into the KP sh cells followed by selection with 10 μg/mL puromycin for 7 days . .. RIPA buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor (PhosSTOP, Roche) were used to lyse the cells.

    Clone Assay:

    Article Title: p53 and YAP/TAZ-TEAD activities determine metaplastic heterogeneity in pancreatic cancer
    Article Snippet: .. LentiCas9-blast selection and pUSEPB selection was performed with 10 μg/mL blasticidin for 7 days and 10 μg/mL puromycin for 7 days respectively. pInducer-20 EGFP-TEADi (Addgene, #140145) was transduced into PDEC and HPAC cells followed by five days of selection using 1 mg/mL geneticin/G418. cDNAs for wildtype and phospho-null YAP and TAZ and EGFP-TEADi were cloned out of pInducer-20 and into the retroviral backbone pBABE-puro for transduction into the KP sh cells followed by selection with 10 μg/mL puromycin for 7 days . .. RIPA buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor (PhosSTOP, Roche) were used to lyse the cells.

    Retroviral:

    Article Title: p53 and YAP/TAZ-TEAD activities determine metaplastic heterogeneity in pancreatic cancer
    Article Snippet: .. LentiCas9-blast selection and pUSEPB selection was performed with 10 μg/mL blasticidin for 7 days and 10 μg/mL puromycin for 7 days respectively. pInducer-20 EGFP-TEADi (Addgene, #140145) was transduced into PDEC and HPAC cells followed by five days of selection using 1 mg/mL geneticin/G418. cDNAs for wildtype and phospho-null YAP and TAZ and EGFP-TEADi were cloned out of pInducer-20 and into the retroviral backbone pBABE-puro for transduction into the KP sh cells followed by selection with 10 μg/mL puromycin for 7 days . .. RIPA buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor (PhosSTOP, Roche) were used to lyse the cells.

    Transduction:

    Article Title: p53 and YAP/TAZ-TEAD activities determine metaplastic heterogeneity in pancreatic cancer
    Article Snippet: .. LentiCas9-blast selection and pUSEPB selection was performed with 10 μg/mL blasticidin for 7 days and 10 μg/mL puromycin for 7 days respectively. pInducer-20 EGFP-TEADi (Addgene, #140145) was transduced into PDEC and HPAC cells followed by five days of selection using 1 mg/mL geneticin/G418. cDNAs for wildtype and phospho-null YAP and TAZ and EGFP-TEADi were cloned out of pInducer-20 and into the retroviral backbone pBABE-puro for transduction into the KP sh cells followed by selection with 10 μg/mL puromycin for 7 days . .. RIPA buffer (Thermo Scientific) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor (PhosSTOP, Roche) were used to lyse the cells.



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    pinducer 20 egfp teadi  (Addgene inc)
    93
    Addgene inc pinducer 20 egfp teadi
    Pinducer 20 Egfp Teadi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pinducer+20+egfp+teadi/pInducer20+EGFP-TEADi+(Plasmid+%23140145)/bio_rxiv__64898__2025__12__11__693520-235-22-24
    Average 93 stars, based on 1 article reviews
    pinducer 20 egfp teadi - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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